Influence of free fatty acid content and degree of fat saturation in laying hen diets on egg quality, yolk fatty acid profile, and cholesterol content

The aim of the present study was to evaluate the effect of dietary free fatty acid (FFA) content and the degree of saturation on egg quality, yolk fatty acid (FA) profile, and yolk cholesterol content. For a 15-wk period, a total of 144 laying hens (19-wk-old) were randomly assigned to 8 treatments arranged in a 2 × 4 factorial design, with 2 sources of crude oil (soybean oil and palm oil) and 4 levels of FFA (10, 20, 30, and 45%). The dietary treatments were achieved by progressively substituting the original oils with equivalent amounts of their corresponding acid oils (soybean acid oil and palm fatty acid distillate, respectively). No differences in ADFI or egg mass were found. However, dietary FFA reduced egg production (linear, P 0.05). The saturation degree had a significant effect on all the analyzed yolk FA (P < 0.001) except for arachidonic acid (C20:4 n-6), whereas increasing the FFA content did not affect to a great extent. These results show that varying dietary FFA level did not affect egg quality and yolk composition as much as the dietary fat source did, supporting the use of acid oils and fatty acid distillates as fat ingredients for feed

Influence of free fatty acid content and degree of fat saturation in laying hen diets on egg quality, yolk fatty acid profile, and cholesterol content

The aim of the present study was to evaluate the effect of dietary free fatty acid (FFA) content and the degree of saturation on egg quality, yolk fatty acid (FA) profile, and yolk cholesterol content. For a 15-wk period, a total of 144 laying hens (19-wk-old) were randomly assigned to 8 treatments arranged in a 2 × 4 factorial design, with 2 sources of crude oil (soybean oil and palm oil) and 4 levels of FFA (10, 20, 30, and 45%). The dietary treatments were achieved by progressively substituting the original oils with equivalent amounts of their corresponding acid oils (soybean acid oil and palm fatty acid distillate, respectively). No differences in ADFI or egg mass were found. However, dietary FFA reduced egg production (linear, P 0.05). The saturation degree had a significant effect on all the analyzed yolk FA (P < 0.001) except for arachidonic acid (C20:4 n-6), whereas increasing the FFA content did not affect to a great extent. These results show that varying dietary FFA level did not affect egg quality and yolk composition as much as the dietary fat source did, supporting the use of acid oils and fatty acid distillates as fat ingredients for feed

Optical And Physical Properties Of Er3+-yb3+ Co-doped Tellurite Fibers

In this work we present results of physical and optical properties of Er3+-Yb3+ co-doped tellurite glasses and fibers. The Double Clad Tellurite Fibers (DCTFs) are based on glasses with the composition: TeO2-WO3-Nb2O5-Na 2O-Al2O3-Er2O3-Yb 2O3. The DCTFs were fabricated by using the rod-in-tube technique and a Heathway drawing tower. The optical absorption spectra (ranging from 350 to 1750 nm) of these fibers were measured using an Optical Spectrum Analyzer (OSA). The emission spectra, around 1550 nm band, of these fibers (lengths varying from 1 to 60 cm) were obtained by using a 980nm diode laser pump. The optimal Amplified Spontaneous Emission (ASE) spectra were observed for fiber lengths ranging from 2 to 6 cm. The Er 3+/Yb3+ co-doped DCTFs show an efficient up-conversion process in comparison with the Er3+-doped DCTF. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).8120The Society of Photo-Optical Instrumentation Engineers (SPIE)Murugan, G.S., Suzuki, T., Ohishi, Y., Raman characteristics and nonlinear optical properties of tellurite and phosphotellurite glasses containing heavy metal oxides with ultrabroad Raman bands (2006) J. of Appl. Phys., 100, p. 023107Nandi, P., Jose, G., Ytterbium-doped P2O5-TeO2 glass for laser applications (2006) IEEE J. of Quat. Electron., 42, pp. 1115-1121Man, S.Q., Pun, E.Y.B., Chung, P.S., Tellurite glasses for 1.3 mm optical amplifiers" (1999) Opt. Comm., 168, pp. 369-373Wang, J.S., Vogel, E.M., Snitzer, E., Tellurite Glass: A New Candidate for Fiber Devices" (1994) Opt. Mat., 3, pp. 187-203Xu, S., Sun, H., Dai, S., Zhang, J., Jiang, Z., Upconversion luminescence of Tm3+/Yb3+-codoped oxyhalide tellurite glasses" (2005) Sol. St. Comm., 133, pp. 89-92Bookey, H.T., Lousteau, J., Jha, A., Gayraud, N., Thomson, R.R., Psaila, N.D., Li, H., Kar, A.K., Multiple rare earth emissions in a multicore tellurite fiber with a single pump wavelength" (2007) Opt. Express, 15, pp. 17554-17561Qin, G., Mori, A., Ohishi, Y., Brillouin lasing in a single-mode tellurite fiber" (2007) Opt. Lett., 32, pp. 2179-2181Zweig, A.D., Frenz, M., Romano, V., Weber, H.P., A comparative study of laser tissue interaction at 2.94 μm and 10.6 μm" (1988) Appl. Phys. B, 47, pp. 259-265Zhang, J., Dai, S., Wang, G., Sun, H., Zhang, L., Hu, L., Fabrication and emission properties of Er3+/Yb3+ codoped tellurite glass fiber for broadband optical amplification (2005) J. of Lum., 115, pp. 45-52Shixun, D., Tiefeng, X., Qiuhua, N., Xiang, S., Xunsi, W., Fabrication and gain performance of Er3+/Yb3+-codoped tellurite glass fiber (2008) J. of Rare Earths, 26, pp. 915-918Jakutis, J., Gomes, L., Amancio, C.T., Kassab, L.R.P., Martinelli, J.R., Wetter, N.U., Increased Er3+ upconversion in tellurite fibers and glasses by co-doping with Yb3+ (2010) Opt. Mat., 33, pp. 107-111Hruby, A., Evaluation of glass-forming tendency by means of DTA (1972) Czech J. Phys B, 22, pp. 1187-119

Innovative passive and active countermeasures for near side crash safety

This research presents six simultaneous innovative occupant near side lateral impact protection concepts including a dynamic door, high-volume side airbag, a large external airbag that covers doors, sill and B-pillar of the struck vehicle and other concepts for increasing the distance between the occupant and the door panel (active armrest, inflatable door beam and moving seat). All systems are based on pre crash detection of the impact and are activated as soon as 80ms before the impact. This paper details the task of integrating these systems into a vehicle using FE models, sled tests, and full scale crash tests. Sled test dummy responses were compared with and without the countermeasures.Postprint (published version

Olive pomace oil and acid oil as alternative fat sources in growing-finishing broiler chicken diets

The aim of the present study was to investigate the effect of dietary supplementation of olive pomace oil and olive pomace acid oil, which are rich in monounsaturated fatty acids (FA) but differ in free FA content, on growth performance, digestibility and FA profile of abdominal fat and breast meat. A total of 3,048 one-day-old mixed-sex broiler chickens (Ross 308) were randomly distributed into 24 pens and 3 dietary treatments (8 replicates per treatment). Experimental diets were administered for growing (from 22 to 29 d) and finishing (from 30 to 39 d) periods, consisting of a basal diet supplemented with 6% (as-fed basis) palm oil (PO), olive pomace oil (O), or olive pomace acid oil (OA). Animals fed O achieved the lowest feed conversion ratio (P < 0.01), together with the highest AME value (P = 0.003), but no differences were observed between OA and PO. Regarding FA digestibility, O and OA showed higher values than PO for all FA in both apparent ileal digestibility (AID) and apparent total tract digestibility. Comparing the AID between O and OA, no differences were observed for total FA, monounsaturated FA, or polyunsaturated FA, but animals fed OA showed lower AID values for saturated FA than those fed O (P < 0.001). The FA profile of abdominal fat and breast meat reflected that of the diet, with higher monounsaturated FA and lower saturated FA in animals fed O and OA compared to those fed PO. In sum, the inclusion of both olive pomace oil and acid oil in growing-finishing broiler chicken diets led to great performance parameters and high FA digestibility values, together with an enrichment with monounsaturated FA in abdominal fat and breast meat compared to the use of palm oil. However, a better AID of saturated FA and feed conversion ratio is achieved with O compared to OA

Olive pomace oil and acid oil as alternative fat sources in growing-finishing broiler chicken diets

The aim of the present study was to investigate the effect of dietary supplementation of olive pomace oil and olive pomace acid oil, which are rich in monounsaturated fatty acids (FA) but differ in free FA content, on growth performance, digestibility and FA profile of abdominal fat and breast meat. A total of 3,048 one-day-old mixed-sex broiler chickens (Ross 308) were randomly distributed into 24 pens and 3 dietary treatments (8 replicates per treatment). Experimental diets were administered for growing (from 22 to 29 d) and finishing (from 30 to 39 d) periods, consisting of a basal diet supplemented with 6% (as-fed basis) palm oil (PO), olive pomace oil (O), or olive pomace acid oil (OA). Animals fed O achieved the lowest feed conversion ratio (P < 0.01), together with the highest AME value (P = 0.003), but no differences were observed between OA and PO. Regarding FA digestibility, O and OA showed higher values than PO for all FA in both apparent ileal digestibility (AID) and apparent total tract digestibility. Comparing the AID between O and OA, no differences were observed for total FA, monounsaturated FA, or polyunsaturated FA, but animals fed OA showed lower AID values for saturated FA than those fed O (P < 0.001). The FA profile of abdominal fat and breast meat reflected that of the diet, with higher monounsaturated FA and lower saturated FA in animals fed O and OA compared to those fed PO. In sum, the inclusion of both olive pomace oil and acid oil in growing-finishing broiler chicken diets led to great performance parameters and high FA digestibility values, together with an enrichment with monounsaturated FA in abdominal fat and breast meat compared to the use of palm oil. However, a better AID of saturated FA and feed conversion ratio is achieved with O compared to OA

Desarrollo de germoplasma de maíz para suelos ácidos: programa suramericano de maíz CIMMYT.

La baja fertilidad de los uselos es el problema más importante en la reducción de los rendimiento de maíz en los tropicos y la acidez del suelo es el principal causante de esta baja fertilidad, siendo la toxicidad de aluminio la principal causa. Actualmente se seimbran entre 8 y 29 millones de ha. de maíz en suelos ácidos con bajo pH, alta concentración de Al, y deficiencia en Ca, Mg y P. En los suelos ácidos se inhibe el crecimiento radicular que finalmente se manifiesta en una dismimunción del rendimiento. El CIMMYT, en colaboración con los Programas Nacionales, esta aprovechando la gran variabilidad en el maíz para generar cultivares que puedan crecer bajo el estress provocado por la ácidez del suelo y que su cultivo sea una actividad económicamente viable y que no interfiera con la sosteniblidad del ambiente. Estudios realizados en Colombia y otros lugares indican que la selección recurrente recíproca, basada en el comportamiento de genotivos a través de un rango de ambiente será efectiva en el desarrollo de variedades e híbridos tolerantes. La capacidad de rendimiento en suelos ácidos esta correlacionada positivamente con el comportamiento en suelos fértiles no ácidos. Por esta razón, los cultivadores de maíz tolerantes a la acidez pueden crecer y produciir bien tanto en suelos ácidos en no ácidos. Como resultado de este proyecto colaborativo se han liberado dos cultivares de maíz: Sikuani ICA V110-Colombia y Antasena en Indonesia, actualmente en evaluación en diferentes países Estudios agronómicos indican la superioridad de los cultivares tolerantes con relación a los susceptibles o comerciales en un rango de suelos ácidos y no ácidosMaíz-Zea may

Image informatics strategies for deciphering neuronal network connectivity

Brain function relies on an intricate network of highly dynamic neuronal connections that rewires dramatically under the impulse of various external cues and pathological conditions. Among the neuronal structures that show morphologi- cal plasticity are neurites, synapses, dendritic spines and even nuclei. This structural remodelling is directly connected with functional changes such as intercellular com- munication and the associated calcium-bursting behaviour. In vitro cultured neu- ronal networks are valuable models for studying these morpho-functional changes. Owing to the automation and standardisation of both image acquisition and image analysis, it has become possible to extract statistically relevant readout from such networks. Here, we focus on the current state-of-the-art in image informatics that enables quantitative microscopic interrogation of neuronal networks. We describe the major correlates of neuronal connectivity and present workflows for analysing them. Finally, we provide an outlook on the challenges that remain to be addressed, and discuss how imaging algorithms can be extended beyond in vitro imaging studies

NeuriteQuant: An open source toolkit for high content screens of neuronal Morphogenesis

<p>Abstract</p> <p>Background</p> <p>To date, some of the most useful and physiologically relevant neuronal cell culture systems, such as high density co-cultures of astrocytes and primary hippocampal neurons, or differentiated stem cell-derived cultures, are characterized by high cell density and partially overlapping cellular structures. Efficient analytical strategies are required to enable rapid, reliable, quantitative analysis of neuronal morphology in these valuable model systems.</p> <p>Results</p> <p>Here we present the development and validation of a novel bioinformatics pipeline called NeuriteQuant. This tool enables fully automated morphological analysis of large-scale image data from neuronal cultures or brain sections that display a high degree of complexity and overlap of neuronal outgrowths. It also provides an efficient web-based tool to review and evaluate the analysis process. In addition to its built-in functionality, NeuriteQuant can be readily extended based on the rich toolset offered by ImageJ and its associated community of developers. As proof of concept we performed automated screens for modulators of neuronal development in cultures of primary neurons and neuronally differentiated P19 stem cells, which demonstrated specific dose-dependent effects on neuronal morphology.</p> <p>Conclusions</p> <p>NeuriteQuant is a freely available open-source tool for the automated analysis and effective review of large-scale high-content screens. It is especially well suited to quantify the effect of experimental manipulations on physiologically relevant neuronal cultures or brain sections that display a high degree of complexity and overlap among neurites or other cellular structures.</p